Cationic dendrimers are super branched polymers with positively charged vertex groups. With the help of these groups, the dendrimer interacts with the molecules of the biological system. The ability of cationic dendrimers to form complexes on the basis of electrostatic interactions ranks dendrimers among suitable non-viral vectors for the transfer of drugs, nucleic acids, and oligonucleotides, and for other possible uses in biomedicine. One nucleic acid that may be suitable for the generation of stable dendriplexes and then for transfection into cells is small interfering ribonucleic acid (siRNA). siRNA regulates cellular expression of genes by RNA interference and is thus one of the potential chances for treatment some of the genetic diseases. Carbosilane dendrimers are suitable candidates for transfer siRNA. Phosphonium carbosilane dendrimers demonstrably form dendriplexes with siRNA. These complexes are formed in different ratios with respect to the positive charge of the surface groups of the carbosilane dendrimer and the negative charge of the siRNA. The obtained dendriplexes of the individual charge ratios were characterized by several instrumental methods such as DLS, zeta potential, electrophoresis, etc.
Anotace v angličtině
Cationic dendrimers are super branched polymers with positively charged vertex groups. With the help of these groups, the dendrimer interacts with the molecules of the biological system. The ability of cationic dendrimers to form complexes on the basis of electrostatic interactions ranks dendrimers among suitable non-viral vectors for the transfer of drugs, nucleic acids, and oligonucleotides, and for other possible uses in biomedicine. One nucleic acid that may be suitable for the generation of stable dendriplexes and then for transfection into cells is small interfering ribonucleic acid (siRNA). siRNA regulates cellular expression of genes by RNA interference and is thus one of the potential chances for treatment some of the genetic diseases. Carbosilane dendrimers are suitable candidates for transfer siRNA. Phosphonium carbosilane dendrimers demonstrably form dendriplexes with siRNA. These complexes are formed in different ratios with respect to the positive charge of the surface groups of the carbosilane dendrimer and the negative charge of the siRNA. The obtained dendriplexes of the individual charge ratios were characterized by several instrumental methods such as DLS, zeta potential, electrophoresis, etc.
Klíčová slova
Fosforiový karbosilanový dendrimer (s periferními substituenty P (Ph) 3), interference RNA, biomedicína, elektroforéza, DLS, zeta potenciál.
Cationic dendrimers are super branched polymers with positively charged vertex groups. With the help of these groups, the dendrimer interacts with the molecules of the biological system. The ability of cationic dendrimers to form complexes on the basis of electrostatic interactions ranks dendrimers among suitable non-viral vectors for the transfer of drugs, nucleic acids, and oligonucleotides, and for other possible uses in biomedicine. One nucleic acid that may be suitable for the generation of stable dendriplexes and then for transfection into cells is small interfering ribonucleic acid (siRNA). siRNA regulates cellular expression of genes by RNA interference and is thus one of the potential chances for treatment some of the genetic diseases. Carbosilane dendrimers are suitable candidates for transfer siRNA. Phosphonium carbosilane dendrimers demonstrably form dendriplexes with siRNA. These complexes are formed in different ratios with respect to the positive charge of the surface groups of the carbosilane dendrimer and the negative charge of the siRNA. The obtained dendriplexes of the individual charge ratios were characterized by several instrumental methods such as DLS, zeta potential, electrophoresis, etc.
Anotace v angličtině
Cationic dendrimers are super branched polymers with positively charged vertex groups. With the help of these groups, the dendrimer interacts with the molecules of the biological system. The ability of cationic dendrimers to form complexes on the basis of electrostatic interactions ranks dendrimers among suitable non-viral vectors for the transfer of drugs, nucleic acids, and oligonucleotides, and for other possible uses in biomedicine. One nucleic acid that may be suitable for the generation of stable dendriplexes and then for transfection into cells is small interfering ribonucleic acid (siRNA). siRNA regulates cellular expression of genes by RNA interference and is thus one of the potential chances for treatment some of the genetic diseases. Carbosilane dendrimers are suitable candidates for transfer siRNA. Phosphonium carbosilane dendrimers demonstrably form dendriplexes with siRNA. These complexes are formed in different ratios with respect to the positive charge of the surface groups of the carbosilane dendrimer and the negative charge of the siRNA. The obtained dendriplexes of the individual charge ratios were characterized by several instrumental methods such as DLS, zeta potential, electrophoresis, etc.
Klíčová slova
Fosforiový karbosilanový dendrimer (s periferními substituenty P (Ph) 3), interference RNA, biomedicína, elektroforéza, DLS, zeta potenciál.
1. Writing the comprehensive review on the topic from already published literature.
2. Preparation and characterization of siRNA-carbosilane dendriplexes with following methods: gel electrophoresis, dynamic light scattering, zeta-potential measurements.
3. Summarization of experimental results, their discussion with already published papers.
Zásady pro vypracování
1. Writing the comprehensive review on the topic from already published literature.
2. Preparation and characterization of siRNA-carbosilane dendriplexes with following methods: gel electrophoresis, dynamic light scattering, zeta-potential measurements.
3. Summarization of experimental results, their discussion with already published papers.
Seznam doporučené literatury
Oliveira, J. M.; Kotobuki, N.; Marques, A. P.; Pirraco, R. P.; Benesch, J.; Hirose, M.; Costa, S. A.; Mano, J. F.; Ohgushi, H.; Reis, R. L., Surface Engineered Carboxymethylchitosan/Poly(amidoamine) Dendrimer Nanoparticles for Intracellular Targeting. Advanced Functional Materials 2008, 18 (12), 1840-1853.
Lesniak, W. G.; Kariapper, M. S.; Nair, B. M.; Tan, W.; Hutson, A.; Balogh, L. P.; Khan, M. K., Synthesis and characterization of PAMAM dendrimer-based multifunctional nanodevices for targeting alphavbeta3 integrins. Bioconjug Chem 2007, 18 (4), 1148-54.
Mather, B.; Viswanathan, K.; Miller, K.; Long, T., Michael addition reactions in macromolecular design for emerging technologies. Progress in Polymer Science 2006, 31 (5), 487-531.
van Baal, I.; Malda, H.; Synowsky, S. A.; van Dongen, J. L.; Hackeng, T. M.; Merkx, M.; Meijer, E. W., Multivalent peptide and protein dendrimers using native chemical ligation. Angew Chem Int Ed Engl 2005, 44 (32), 5052-7.
Svenson, S.; Tomalia, D. A., Dendrimers in biomedical applications--reflections on the field. Adv Drug Deliv Rev 2005, 57 (15), 2106-29.
Ackerson, C. J.; Sykes, M. T.; Kornberg, R. D., Defined DNA/nanoparticle conjugates. Proceedings of the National Academy of Sciences of the United States of America 2005, 102 (38), 13383-5.
Pedziwiatr-Werbicka, E., SHCHARBIN, D., MALY, J. et al. Carbosilane Dendrimers are a Non-Viral Delivery System for Antisense Oligonucleotides: Characterization of Dendriplexes. Journal of Biomedical Nanotechnology. 2012, 8(1), 57-73. DOI: 10.1166/jbn.2012.1369. ISSN 15507033.
Biwas, S., Torchilin, V., Dendrimers for delivery. Pharmaceuticals 2013, 6, 161-183; doi:10.3390/ph6020161
Ferenz, M., Pedziwiatr-Werbicka, E., , Nowak, K., Klajnert, B., Majoral, J.P., Bryszewska, M. Phosphorus Dendrimers as Carriers of siRNACharacterisation of Dendriplexes. Molecules. 2013, 18(4), 4451-4466. DOI: 10.3390/molecules18044451. ISSN 1420-3049.
Shcharbin, D., Pedziwiatr, E., Bryszewska, M. How to study dendriplexes I: Characterization. Journal of Controlled Release. 2009, 135(3), 186-197. DOI: 10.1016/j.jconrel.2009.01.015.
ISSN 01683659.
Shcharbin, D., Pedziwiatr, E., Blasiak, J., Bryszewska. How to study dendriplexes II: Transfection and cytotoxicity. Journal of Controlled Release 2010, 141(2), 110-127. DOI:10.1016/j.jconrel.2009.09.030. ISSN 01683659.
Biricova, V. Laznickova, A. Dendrimers: Analytical characterization and applications. Bioorganic Chemistry. 2009, 37(6), 185-192. DOI: 10.1016/j.bioorg.2009.07.006. ISSN 00452068.
Seznam doporučené literatury
Oliveira, J. M.; Kotobuki, N.; Marques, A. P.; Pirraco, R. P.; Benesch, J.; Hirose, M.; Costa, S. A.; Mano, J. F.; Ohgushi, H.; Reis, R. L., Surface Engineered Carboxymethylchitosan/Poly(amidoamine) Dendrimer Nanoparticles for Intracellular Targeting. Advanced Functional Materials 2008, 18 (12), 1840-1853.
Lesniak, W. G.; Kariapper, M. S.; Nair, B. M.; Tan, W.; Hutson, A.; Balogh, L. P.; Khan, M. K., Synthesis and characterization of PAMAM dendrimer-based multifunctional nanodevices for targeting alphavbeta3 integrins. Bioconjug Chem 2007, 18 (4), 1148-54.
Mather, B.; Viswanathan, K.; Miller, K.; Long, T., Michael addition reactions in macromolecular design for emerging technologies. Progress in Polymer Science 2006, 31 (5), 487-531.
van Baal, I.; Malda, H.; Synowsky, S. A.; van Dongen, J. L.; Hackeng, T. M.; Merkx, M.; Meijer, E. W., Multivalent peptide and protein dendrimers using native chemical ligation. Angew Chem Int Ed Engl 2005, 44 (32), 5052-7.
Svenson, S.; Tomalia, D. A., Dendrimers in biomedical applications--reflections on the field. Adv Drug Deliv Rev 2005, 57 (15), 2106-29.
Ackerson, C. J.; Sykes, M. T.; Kornberg, R. D., Defined DNA/nanoparticle conjugates. Proceedings of the National Academy of Sciences of the United States of America 2005, 102 (38), 13383-5.
Pedziwiatr-Werbicka, E., SHCHARBIN, D., MALY, J. et al. Carbosilane Dendrimers are a Non-Viral Delivery System for Antisense Oligonucleotides: Characterization of Dendriplexes. Journal of Biomedical Nanotechnology. 2012, 8(1), 57-73. DOI: 10.1166/jbn.2012.1369. ISSN 15507033.
Biwas, S., Torchilin, V., Dendrimers for delivery. Pharmaceuticals 2013, 6, 161-183; doi:10.3390/ph6020161
Ferenz, M., Pedziwiatr-Werbicka, E., , Nowak, K., Klajnert, B., Majoral, J.P., Bryszewska, M. Phosphorus Dendrimers as Carriers of siRNACharacterisation of Dendriplexes. Molecules. 2013, 18(4), 4451-4466. DOI: 10.3390/molecules18044451. ISSN 1420-3049.
Shcharbin, D., Pedziwiatr, E., Bryszewska, M. How to study dendriplexes I: Characterization. Journal of Controlled Release. 2009, 135(3), 186-197. DOI: 10.1016/j.jconrel.2009.01.015.
ISSN 01683659.
Shcharbin, D., Pedziwiatr, E., Blasiak, J., Bryszewska. How to study dendriplexes II: Transfection and cytotoxicity. Journal of Controlled Release 2010, 141(2), 110-127. DOI:10.1016/j.jconrel.2009.09.030. ISSN 01683659.
Biricova, V. Laznickova, A. Dendrimers: Analytical characterization and applications. Bioorganic Chemistry. 2009, 37(6), 185-192. DOI: 10.1016/j.bioorg.2009.07.006. ISSN 00452068.