The course objective is to comprehensively understand the influence of nanomaterials on biological systems such as lipid membranes and proteins, employing both theoretical analysis and empirical exploration. This insight is pivotal for advancing applications in medicine, drug delivery, diagnostics, and other pertinent domains, necessitating an in-depth study of nanoparticle behaviour at molecular and cellular tiers. Moreover, the profound comprehension of nanoparticle architecture and its ensuing implications holds promise for the innovation of more efficient biological systems. Throughout the course, students will also gain practical experience in utilizing cutting-edge laboratory tools for characterizing nanomaterials in biological contexts, thereby equipping them with valuable skills for future research and applications. This course combines theoretical learning with hands-on training. The main goal is to promote and enhance students' competence in using advanced biophysical and biochemical techniques efficiently. Students will achieve the following skills: Biophysical nanomaterials characterization: Students will obtain knowledge about the construction of scientific investigation of new nanomaterials as well as biophysical methods used in this area. Interactions with biological structures: Students will obtain knowledge about the preparation of lipid bilayer model systems and their usage in lipid membranes behaviour investigation, they will also research the influence on proteins of nanoparticles. Analytical Proficiency: Students will develop a strong foundation in analytical techniques, allowing them to accurately assess and interpret complex biophysical and biochemical data. This skill is essential for making informed scientific conclusions based on experimental results. Interdisciplinary Integration: The course will emphasize the integration of principles from biology, physics, chemistry, and other related fields. Students will learn how to synthesize knowledge from various disciplines to gain a holistic understanding of complex biological phenomena. Problem-Solving Skills: Biological systems can present intricate challenges. Through the course, students will develop problem-solving skills to address research questions, troubleshoot experimental issues, and adapt methodologies as needed. Students will acquire comprehensive insights into the operational principles and application methodologies of cutting-edge analytical instruments. These instruments encompass a spectrofluorimeter, circular dichroism spectrometer, nanosizer, micro-isothermal titration calorimeter (micro-ITC), micro-differential scanning calorimeter (micro-DSC), and the sophisticated nanoparticle tracking analyzer (NTA). Moreover, students will undergo rigorous training in the utilization of specialized software tools for the meticulous analysis of acquired data. Notably, proficiency in the employment of GraphPad Prism7 for data interpretation and statistical analysis will be cultivated. Furthermore, the course will impart advanced knowledge and practical skills pertinent to the construction and execution of intricate analyses concerning novel nanoparticles. Students will become adept at delineating the multifaceted characteristics of these nanoparticles, thereby nurturing their capacity for innovative scientific inquiry within this burgeoning field. Practical part of the course Preparation and characterization of lipid-bilayer and proteins models. Investigation of nanoparticles interaction with lipid-bilayer and proteins models by several biophysical methods. Theoretical part of the course
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