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Lecturer(s)
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Čejnová Jana, Mgr.
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Štolba Petr, RNDr.
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Liegertová Michaela, Mgr. Ph.D.
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Course content
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1. model organisms (history, basic terminology) 2. E. coli (basics of cloning, recombinant DNA technology) 3. Yeasts (2-hybrid system) 4. C. elegans (RNAi) 5. Insects (P-element transformation, Gal4/UAS system) 6. P. dumerilii (gene expression profiling in cellular resolution) 7. Lancelets (RNA in situ/FISH) 8. Fish (I-SceI transgenesis, Zn-finger nucleases, TALENs) 9. Amphibians (morpholino knock-down) 10. G. domesticus (viral transgenesis) 11. M. musculus (ES cells modifications, Cre-LoxP system, CRISPRs system) 12. Basics of Bioinformatics (+ practical exercise)
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Learning activities and teaching methods
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unspecified
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Learning outcomes
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Prerequisites
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Students will learn and understand basic terminology, approaches and methods used in molecular genetics and genetic engineering.
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Assessment methods and criteria
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unspecified
Students are expected to learn and understand basic terminology, approaches and methods used in modern molecular genetics and genetic engineering.
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Recommended literature
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ALBERTS, Bruce, Alexandr JOHNSON a Julian LEWIS, et al. Molecular Biology of the Cell. 2015 (ev. některé předchozí vydání).
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Bruce A. Molecular Biology of the Cell.
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Cvrčková Fatima. Úvod do praktické bioinformatiky, Academia, (2006).
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Dorian J., et al. Základy lékařské genetiky.. Praha: Galén, 2007. ISBN 978-80-7262-449-2.
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Gilbert S. Developmental Biology.
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Gilbert S. F. Developmental Biology. 10th ed. Sinauer Associates, Inc., 2013..
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KOČÁREK, E. et al. Molekulární biologie v medicíně. Praha: NCO NZO, 2007. 218 s. ISBN 978-80-7013-450-4.
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Rosypal, S. Úvod do molekulární biologie. Brno,. 1997.
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ŠMARDA, J. a kol. Metody molekulární biologie. Masarykova univerzita, Brno, 2010. ISBN 978-8-0210-3841-7.
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Watson J.D., Baker T.A., Bell S.P., Gann A. Molecular Biology of the Gene. 7th ed. Benjamin Cummings, 2013..
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