1. Plant genomes. Organisation of plant nuclear, mitochondrial, plasmid genome. Domestication of crops. Dometication traits, domestication syndrome. 2. Conventional methods of breeding, special methods of plant cultivation. Cryopreservation. 3. Tissue cultures. Aseptic cultures of plants, embryos, organs, cells, protoplasts. Regeneration, morphogenesis. Stability of tissue cultures. Types of media, hormones, incubation. 4. Callus, haploid cultures, practical use. Somatoclonal variability, somatic embryogenesis. Micropropagation. Suspension cultures. 5. Methods of GMO preparation, transformarion of plant cells. Direct and non-direct methods of gene delivery. Particle gun bombardment, microinjection, electroporation, sonication, Bacterial, viral vectors. Agrobacterium tumefaciens, Ti plasmid. 6. Transgenic plants 1. Transgenes against herbicides, insecticides. GMO for high yield. 7. Transgenic plants 2. Transgenes for production of secondary metabolites. Molecular farming. Biofortification. 8. GMO legislative, GMO potential, risks. 9. Phytoremediation, bioremediation.
|
-
Altman A., Hasegawa, P.M. Plant Biotechnology and Agriculture: Prospects for the 21st Century. 1st ed. Academic Press, 2011..
-
Lindsey K. Plant tissue culture. Manual. Kluwer Academic Publishers, London, 1992.
-
Slater A., Scott N., Fowler M. Plant biotechnology: the genetic manipulation of plants. 2nd ed. Oxford: Oxford University Press, 2003..
-
Smith R. H. Plant tissue culture. Techniques and experiments. Second edition. Academic Press, 2000.
|