JAN EVANGELISTA PURKYNĚ UNIVERSITY IN ÚSTÍ NAD LABEM
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JAN EVANGELISTA PURKYNĚ UNIVERSITY IN ÚSTÍ NAD LABEM
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Course: Electron microscopy in biology

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Course title Electron microscopy in biology
Course code KBI/N027
Organizational form of instruction Lesson
Level of course Master
Year of study not specified
Semester Summer
Number of ECTS credits 3
Language of instruction Czech
Status of course Compulsory
Form of instruction Face-to-face
Work placements This is not an internship
Recommended optional programme components None
Lecturer(s)
  • Benada Oldřich, RNDr. CSc.
Course content
The first part of the course will be devoted to acquiring fundamental information and skills in Transmission electron microscopy (TEM) and Scanning electron microscopy (SEM) in biology. The second part will include sample preparation techniques and methods used in biological electron microscopy. The third part will include the strategies of device optimization and image data recording in TEM and SEM. An image data storing and processing will be discussed as well. Based on the above mentioned skills each student will choose a project to complete and submit formal written and oral reports of their findings.

Learning activities and teaching methods
unspecified, unspecified, unspecified
Learning outcomes
Students should learn the basic differences between transmission and scanning electron microscopes and the technique of digital image recording in TEM and SEM, image processing and archiving of image data sets. Students also should get theoretical knowledge of biological sample preparation for TEM and SEM and also pass through some practical training in preparation of selected samples for electron microscopy.

Prerequisites
unspecified

Assessment methods and criteria
unspecified
Recommended literature
  • Bartl, P.; Delong, A.; Drahoš, V.; Hrivňák, I. & Rosenberg. ), Metody elektronové mikroskopie, Nakladatelství Československé akademie věd, Praha 1964..
  • Edited by M. A. Nasser Hajibagheri. Electron microscopy methods and protocols. Totowa, NJ, USA: Humana Press, 1999. pp.283 (paper)..
  • Joseph Goldstein, Dale E. Newbury, David C. Joy, Charles E. Lyman, Patrick Echlin, Eric Lifshin, Linda C. Sawyer, J.R. Michael (Eds.). Scanning Electron Microscopy and X-Ray Microanalysis. Kluwer Academic/Plenum Publishers, New York/Boston/Dordrecht/London/Moscow, 2003, pp. 689.
  • Kalina T., Pokorný V. Základy elektronové mikroskopie pro biology.. PřF UK Praha, 1981.
  • Ludvík, J. Vybrané metody v mikrobiologii, Academia Praha, Cudlín, J., ed., kapitola Elektronová mikroskopie, pp. 54-104.. 1981.
  • Michael J. Dykstra, Laura E Reuss. Biological Electron Microscopy - Theory, Techniques, and Troubleshooting - Second Edition. Kluwer Academic/Plenum December 2003, pp. 534.
  • Proser, V. & kolektiv. Experimentální metody biofyziky, Academia Praha, kapitola Elektronový mikroskop, pp. 276-313. 1989.


Study plans that include the course
Faculty Study plan (Version) Category of Branch/Specialization Recommended year of study Recommended semester
Faculty: Faculty of Science Study plan (Version): Biology (A16) Category: Biology courses 1 Recommended year of study:1, Recommended semester: Summer
JAN EVANGELISTA PURKYNĚ UNIVERSITY IN ÚSTÍ NAD LABEM, date of update: 01.06.2024 23:51.