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Lecturer(s)
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Auer Malinská Hana, Mgr. Ph.D.
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Czerneková Michaela, Mgr. Ph.D.
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Liegertová Michaela, Mgr. Ph.D.
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Wróbel Dominika Zofia, Ph.D.
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Course content
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Each of the topics usually involves 1 week of theoretical preparation and 1 week of practical laboratory experiment. Topics: 1) Good laboratory practice - Safety rules and regulations 2) in vitro cell cultures - plants Nicotiana tabacum, Solanum tuberosum, Daucus carota), plant cultures, callus cultures, suspension cultures 3) in vitro cell cultures - animals/human (mammalian cell and human carcinoma cell lines) 4) in vivo models - plants (Nicotiana tabacum, Miscanthus x giganteus, model for phytoremediation, stress physiology), Pisum sativum -salinity stress testing 5) in vivo models - invertebrates (Tardigrades as an emerging model for cryptobiosis, pharmaceutics and cellular biology) 6) in vivo models - vertebrates (Danio rerio - toxicity testing, xenotransplantation, animal welfare and legal issues)
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Learning activities and teaching methods
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unspecified
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Learning outcomes
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In this specialized course, the students will get a practical introduction into basic in vitro and in vivo experimental techniques in biology. These include work with plant and animal/human cell cultures (2D/3D) as well as experimental work with living organisms (plants, invertebrates, vertebrates). Particular emphasis will be laid on the design and planning of experimental work, documentation, critical evaluation including qualitative and quantitative analysis of the results, as well as laboratory safety and ethical aspects.
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Prerequisites
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unspecified
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Assessment methods and criteria
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unspecified
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Recommended literature
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