| Course title | Introduction to Scanning Electron Microscopy in Biology |
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| Course code | KBI/E114 |
| Organizational form of instruction | Lesson |
| Level of course | unspecified |
| Year of study | not specified |
| Semester | Winter and summer |
| Number of ECTS credits | 5 |
| Language of instruction | English |
| Status of course | unspecified |
| Form of instruction | Face-to-face |
| Work placements | This is not an internship |
| Recommended optional programme components | None |
| Lecturer(s) |
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| Course content |
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1) Introduction: good laboratory practice in molecular genetics, designing the experiments 2) Laboratory exercise 1 - Good laboratory practice 3) Extraction and purification of DNA from microbial cultures (theory), polymerase chain reaction (PCR) use in molecular genetic detection methods (theory), selective DNA primers 4) Laboratory exercise 2 - Extraction and purification of environmental and microbial DNA, PCR 5) Purification of the PCR products, gel electrophoresis, restriction cleavage (theory), gradient gel electrophoresis DGGE and TGGE 6) Laboratory exercise 3 - Purification of the PCR products, gel electrophoresis 7) Methods of the amplicon analysis: RFLP, T-RFLP, RAPD. Ligation of DNA fragments, AFLP 8) Laboratory exercise 4 - Restriction analysis 9) DNA sequencing: Sanger sequencing and high-throughput sequencing methods. The use of sequencing methods in the identification of microorganisms 10) Analysis of sequencing data in microbial ecology 11) Molecular cloning, cloning vectors, heterologous gene expression, expression vectors for E. coli and yeasts. 12) Examples of molecular genetic analysis of microbial communities I. 13) Examples of molecular genetic analysis of microbial communities II.
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| Learning activities and teaching methods |
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| Learning outcomes |
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Students should learn the basic differences between transmission and scanning electron microscopes and the technique of digital image recording in TEM and SEM, image processing and archiving of image data sets. Students also should get theoretical knowledge of biological sample preparation for TEM and SEM and also pass through some practical training in preparation of selected samples for electron microscopy.
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| Prerequisites |
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unspecified
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| Assessment methods and criteria |
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unspecified
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| Recommended literature |
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| Study plans that include the course |
| Faculty | Study plan (Version) | Category of Branch/Specialization | Recommended semester |
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