|
Lecturer(s)
|
|
|
|
Course content
|
1. Introduction to biosensors - Definition of biosensor, elementary parts and functions, criteria of biosensor function, conditions of measurements with biosensors 2. Nature and fiction of transducers - Pontentiometric, amperometric, conductometric systems, optical biosensors, bioaffinity based biosensors - surface plasmons, piezoelectric systems, kinetics of affinity reactions 3. Nature and function of biological part of biosensor - Whole cell biosensors, enzyme electrodes, immunosensors, DNA sensors 4. Immobilization of biomolecules - Covalent, affinity and nonspecific immobilization, multi and monolayer immobilization, electropolymerization 5. Biochips - Optical and electrochemical biochips, DNA and protein biochips, nanotechnology and nanobiotechnology
|
|
Learning activities and teaching methods
|
|
unspecified
|
|
Learning outcomes
|
Research in the field of biosensors represents a fast growing interdisciplinary science, which gather the knowledge from biology, chemistry, physics and material science. It may potentially brings many important analytical and bioanalytical applications in the medicine, pharmacy, environmental and pollution control, control of food quality, agriculture etc. As the results of ongoing miniaturization of biosensors, this research field is closely tight to micro- and nanotechnological science and to more recently developing field of nanobiotechnology. The basic goal of the subject is to introduce students in the basic principles of biosensors in the theoretical and practical part and to give them an overview in the rapidly growing and interconnecting bioanalytical fields.
|
|
Prerequisites
|
unspecified
|
|
Assessment methods and criteria
|
unspecified
|
|
Recommended literature
|
-
Allen J. Bard, Larry R. Faulkner. Electrochemical Methods.. Fundamentals and Applications John Wiley and Sons, 2000.
-
Cooper J., Cass T. Biosensors - a practical approach.. Oxford University Press, 2004.
-
Dostál J., Pavlová H., Slanina J., Táborská E. Biochemie pro bakaláře, LF MU Brno, 2003. 2003.
-
Skládal P. Biosenzory.. PřF MU, 2002.
|